CoraLite®594-conjugated STAT2 Monoclonal antibody
STAT2 Monoclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1G12C4
验证数据展示
经过测试的应用
Positive IF detected in | Ramos cells |
Positive FC detected in | Ramos cells |
Planning an IHC experiment? We recommend our IHCeasy STAT2 Ready-To-Use IHC Kit. STAT2 primary antibody included.
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66485 targets STAT2 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | FC (Intra), IF/ICC |
Tested Reactivity | Human, mouse, rat |
Immunogen | STAT2 fusion protein Ag10168 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | signal transducer and activator of transcription 2, 113kDa |
Synonyms | ISGF 3, P113, STAT113, STAT2 |
Calculated Molecular Weight | 851 aa, 98 kDa |
Observed Molecular Weight | 113 kDa |
GenBank Accession Number | BC051284 |
Gene Symbol | STAT2 |
Gene ID (NCBI) | 6773 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P52630 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STAT2, also named as p113, belongs to the transcription factor STAT family. It is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IF stimulated genes, which drive the cell in an antiviral state. It also interacts with CRSP2, CRSP6, Simian virus 5 protein V, rabies virus phosphoprotein, IFNAR1 and IFNAR2. Its interaction with dengue virus NS5 inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), STAT2 is targeted for degradation. The calculated molecular weight of STAT2 is 98 kDa, but phosphorylated STAT2 is about 100-113 kDa.
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL594 STAT2 antibody CL594-66485 | Download protocol |
FC protocol for CL594 STAT2 antibody CL594-66485 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |