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验证数据展示

  • WB analysis using 85131-4-RR

    WB analysis using 85131-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85131-4-RR (STARD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 85131-4-RR

    IHC staining of human colon cancer using 85131-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85131-4-RR (STARD7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 85131-4-RR

    IHC staining of human colon cancer using 85131-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85131-4-RR (STARD7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 85131-4-RR

    IHC staining of human colon cancer using 85131-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85131-4-RR (STARD7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85131-4-RR

    Affinity and Kinetic Characterization of 85131-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85131-4-RR against Human STARD7 were performed. The affinity constant is below 1 pM.

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STARD7 Recombinant monoclonal antibody

STARD7 Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85131-4-RR

产品说明书

CloneNo. 242760A3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

242760A3, GTT1, StAR-related lipid transfer protein 7, mitochondrial, START domain-containing protein 7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HT-1080 cells, MCF-7 cells, K-562 cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85131-4-RR targets STARD7 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8224

Product name: Recombinant human STARD7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-295 aa of BC008894

Sequence: MAALAGVFVWDEERIQEEELQRSINEMKRLEEMSNMFQSSGVQHHPPEPKAQTEGNEDSEGKEQRWEMVMDKKHFKLWRRPITGTHLYQYRVFGTYTDVTPRQFFNVQLDTEYRKKWDALVIKLEVIERDVVSGSEVLHWVTHFPYPMYSRDYVYVRRYSVDQENNMMVLVSRAVEHPSVPESPEFVRVRSYESQMVIRPHKSFDENGFDYLLTYSDNPQTVFPRYCVSWMVSSGMPDFLEKLHMATLKAKNMEIKVKDYISAKPLEMSSEAKATSQSSERKNEGSCGPARIEYA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 StAR-related lipid transfer (START) domain containing 7
别名 242760A3, GTT1, StAR-related lipid transfer protein 7, mitochondrial, START domain-containing protein 7
计算分子量 295 aa, 35 kDa
观测分子量 34 kDa
GenBank蛋白编号BC008894
基因名称 STARD7
Gene ID (NCBI) 56910
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NQZ5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STARD7, also known as gestational trophoblastic tumor gene-1 (GTT1), is a lipid transfer protein that specifically binds and transports phosphatidylcholine (PC) between cellular membranes. It is a member of the START (Steroidogenic Acute Regulatory protein-related lipid transfer) domain-containing protein family, which is involved in intracellular lipid transport and metabolism. (PMID: 23507753)

实验方案

Product Specific Protocols
WB protocol for STARD7 antibody 85131-4-RRDownload protocol
IHC protocol for STARD7 antibody 85131-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols