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验证数据展示

  • WB analysis of mouse liver using 10487-1-AP

    WB analysis of mouse liver using 10487-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10487-1-AP (STARD5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 10487-1-AP

    WB analysis using 10487-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10487-1-AP (STARD5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 10487-1-AP

    IHC staining of human liver using 10487-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 10487-1-AP (STARD5 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 10487-1-AP

    IF Staining of HepG2 using 10487-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using STARD5 antibody (10487-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

STARD5 Polyclonal antibody

STARD5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10487-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

START domain-containing protein 5, StAR-related lipid transfer protein 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells, mouse liver tissue, HEK-293T cells, HepG2 cells, THP-1 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

10487-1-AP targets STARD5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 STARD5 fusion protein Ag0753 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 StAR-related lipid transfer (START) domain containing 5
别名 START domain-containing protein 5, StAR-related lipid transfer protein 5
计算分子量 23.4 kDa
观测分子量 24-26 kDa
GenBank蛋白编号BC004365
基因名称 STARD5
Gene ID (NCBI) 80765
RRIDAB_2197808
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NSY2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for STARD5 antibody 10487-1-APDownload protocol
IHC protocol for STARD5 antibody 10487-1-APDownload protocol
IF protocol for STARD5 antibody 10487-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

PLoS One

Relationships between hematopoiesis and hepatogenesis in the midtrimester fetal liver characterized by dynamic transcriptomic and proteomic profiles.

Authors - Yuanbiao Guo
View Article
humanWB

Int J Oncol

AUP1 transcriptionally activated by KDM5B reprograms lipid metabolism to promote the malignant progression of cervical cancer

Authors - Yingping Zhu
View Article