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验证数据展示

  • WB analysis using 20502-1-AP

    WB analysis using 20502-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20502-1-AP (STARD3NL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 20502-1-AP

    IHC staining of human breast cancer using 20502-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20502-1-AP (STARD3NL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 20502-1-AP

    IHC staining of human breast cancer using 20502-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20502-1-AP (STARD3NL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HEK-293 using 20502-1-AP

    IP experiment of HEK-293 using 20502-1-AP

    IP result of anti-STARD3NL (IP:20502-1-AP, 4ug; Detection:20502-1-AP 1:500) with HEK-293 cells lysate 3200ug.

  • IF Staining of U2OS using 20502-1-AP

    IF Staining of U2OS using 20502-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using STARD3NL antibody (20502-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO验证

STARD3NL Polyclonal antibody

STARD3NL Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20502-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

MENTHO, STARD3 N terminal like, STARD3 N terminal like protein, STARD3NL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU2OS cells, HEK-293 cells, mouse kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20502-1-AP targets STARD3NL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 STARD3NL fusion protein Ag14273 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 STARD3 N-terminal like
别名 MENTHO, STARD3 N terminal like, STARD3 N terminal like protein, STARD3NL
计算分子量 234 aa, 27 kDa
观测分子量 27 kDa
GenBank蛋白编号BC005959
基因名称 STARD3NL
Gene ID (NCBI) 83930
RRIDAB_10694281
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95772
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for STARD3NL antibody 20502-1-APDownload protocol
IHC protocol for STARD3NL antibody 20502-1-APDownload protocol
IF protocol for STARD3NL antibody 20502-1-APDownload protocol
IP protocol for STARD3NL antibody 20502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

J Cell Mol Med

STARD3NL inhibits the osteogenic differentiation by inactivating the Wnt/β-catenin pathway via binding to Annexin A2 in osteoporosis.

Authors - Yuexin Xu
  • KD Validated
View Article