验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HeLa cells |
| Positive IHC detected in | human stomach cancer tissue, mouse stomach tissue, rat stomach tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells, A549 cells |
Planning an IHC experiment? We recommend our IHCeasy® STARD13 Ready-To-Use IHC Kit. STARD13 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
产品信息
21325-1-AP targets STARD13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag15012 Product name: Recombinant human STARD13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 113-393 aa of BC046563 Sequence: LDVNFQRKKGDDSDEEDLCISNKWTFQRTSRRWSRVDDLYTLLPRGDRNGSPGGTGMRNTTSSESVLTDLSEPEVCSIHSESSGGSDSRSQPGQCCTDNPVMLDAPLVSSSLPQPPRDVLNHPFHPKNEKPTRARAKSFLKRMETLRGKGAHGRHKGSGRTGGLVISGPMLQQEPESFKAMQCIQIPNGDLQNSPPPACRKGLPCSGKSSGESSPSEHSSSGVSTPCLKERKCHEANKRGGMYLEDLDVLAGTALPDAGDQSRMHEFHSQENLVVHIPKDH 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | StAR-related lipid transfer (START) domain containing 13 |
| 别名 | 46H23.2, DLC 2, DLC2, DLC-2, GT650 |
| 计算分子量 | 1113 aa, 125 kDa |
| 观测分子量 | 125 kDa |
| GenBank蛋白编号 | BC046563 |
| 基因名称 | STARD13 |
| Gene ID (NCBI) | 90627 |
| RRID | AB_2878840 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y3M8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for STARD13 antibody 21325-1-AP | Download protocol |
| IHC protocol for STARD13 antibody 21325-1-AP | Download protocol |
| WB protocol for STARD13 antibody 21325-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Biol Lett LncRNA-like MMP14 RNA facilitates colorectal cancer metastasis by suppressing H3K27cr at the STARD13 promoter region. | ||
Life Sci Role of exosomal microRNA-125b-5p in conferring the metastatic phenotype among pancreatic cancer cells with different potential of metastasis. | ||
Biol Proced Online Circ_0003570 Suppresses the progression of hepatocellular carcinoma through miR-182-5p/STARD13 regulatory axis | ||
Endocrine Loss of STARD13 contributes to aggressive phenotype transformation and poor prognosis in papillary thyroid carcinoma
| ||
Environ Toxicol MiR-125b enhances doxorubicin-induced cardiotoxicity by suppressing the nucleus-cytoplasmic translocation of YAP via targeting STARD13. |
