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验证数据展示

  • WB analysis using 17048-1-AP

    WB analysis using 17048-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17048-1-AP (STARD10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 17048-1-AP

    IHC staining of human liver using 17048-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17048-1-AP (STARD10 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 17048-1-AP

    IHC staining of human liver using 17048-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17048-1-AP (STARD10 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of HepG2 using 17048-1-AP

    IP experiment of HepG2 using 17048-1-AP

    IP result of anti-STARD10 (IP:17048-1-AP, 4ug; Detection:17048-1-AP 1:300) with HepG2 cells lysate 3400 ug.

STARD10 Polyclonal antibody

STARD10 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 17048-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, IHC, ELISA

别名

Antigen NY CO 28, CGI 52, NY CO 28, PCTP L, PCTP like protein, PCTP2, SDCCAG28, STARD10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, T-47D cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17048-1-AP targets STARD10 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag10705

Product name: Recombinant human STARD10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-291 aa of BC007919

Sequence: MEKLAASTEPQGPRPVLGRESVQVPDDQDFRSFRSECEAEVGWNLTYSRAGVSVWVQAVEMDRTLHKIKCRMECCDVPAETLYDVLHDIEYRKKWDSNVIETFDIARLTVNADVGYYSWRCPKPLKNRDVITLRSWLPMGADYIIMNYSVKHPKYPPRKDLVRAVSIQTGYLIQSTGPKSCVITYLAQVDPKGSLPKWVVNKSSQFLAPKAMKKMYKACLKYPEWKQKHLPHFKPWLHPEQSPLPSLALSELSVQHADSLENIDESAVAESREERMGGAGGEGSDDDTSLT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 StAR-related lipid transfer (START) domain containing 10
别名 Antigen NY CO 28, CGI 52, NY CO 28, PCTP L, PCTP like protein, PCTP2, SDCCAG28, STARD10
计算分子量 291 aa, 33 kDa
观测分子量35-40 kDa
GenBank蛋白编号BC007919
基因名称 STARD10
Gene ID (NCBI) 10809
RRIDAB_2255485
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y365
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STARD10 (START domain-containing protein 10, also known as PCTP-like protein) is highly expressed in liver and responsible to transfer phosphatidylcholine. STARD10 was identified as a protein overexpressed in breast cancer that cooperates with the ErbB family of receptor tyrosine kinases in cellular transformation (PMID: 15911624). This antibody detected duplicate bands between 35-40 kDa (PMID: 15150109).

实验方案

Product Specific Protocols
IHC protocol for STARD10 antibody 17048-1-APDownload protocol
IP protocol for STARD10 antibody 17048-1-APDownload protocol
WB protocol for STARD10 antibody 17048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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