验证数据展示
经过测试的应用
Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
Positive IHC detected in | human gliomas tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse skeletal muscle tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:14000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20392-1-AP targets STAC3 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF-P, ELISA Application Description |
文献引用应用 | WB, IHC, IF, CoIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | STAC3 fusion protein Ag14232 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | SH3 and cysteine rich domain 3 |
别名 | SH3 and cysteine-rich domain-containing protein 3, SH3 and cysteine rich domain 3 |
计算分子量 | 364 aa, 42 kDa |
观测分子量 | 42-55 kDa |
GenBank蛋白编号 | BC008069 |
基因名称 | STAC3 |
Gene ID (NCBI) | 246329 |
RRID | AB_10693618 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q96MF2 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for STAC3 antibody 20392-1-AP | Download protocol |
IHC protocol for STAC3 antibody 20392-1-AP | Download protocol |
IF protocol for STAC3 antibody 20392-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Hum Mutat STAC3 variants cause a congenital myopathy with distinctive dysmorphic features and malignant hyperthermia susceptibility. | ||
Structure Multiple Sequence Variants in STAC3 Affect Interactions with CaV1.1 and Excitation-Contraction Coupling.
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Skelet Muscle The SH3 and cysteine-rich domain 3 (Stac3) gene is important to growth, fiber composition, and calcium release from the sarcoplasmic reticulum in postnatal skeletal muscle.
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J Cell Physiol STAC3 incorporation into skeletal muscle triads occurs independent of the dihydropyridine receptor. | ||
J Gen Physiol Calcium current modulation by the γ1 subunit depends on alternative splicing of CaV1.1. | ||
J Appl Physiol (1985) Preconditioning contractions prevent prolonged force depression and Ca2+-dependent proteolysis of STAC3 after damaging eccentric contractions. |