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STAC3 Polyclonal antibody

STAC3 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 20392-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA and More (2)

SH3 and cysteine-rich domain-containing protein 3, SH3 and cysteine rich domain 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman gliomas tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:14000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20392-1-AP targets STAC3 in WB, IHC, IF-P, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 STAC3 fusion protein Ag14232 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH3 and cysteine rich domain 3
别名 SH3 and cysteine-rich domain-containing protein 3, SH3 and cysteine rich domain 3
计算分子量 364 aa, 42 kDa
观测分子量 42-55 kDa
GenBank蛋白编号BC008069
基因名称 STAC3
Gene ID (NCBI) 246329
RRIDAB_10693618
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96MF2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for STAC3 antibody 20392-1-APDownload protocol
IHC protocol for STAC3 antibody 20392-1-APDownload protocol
IF protocol for STAC3 antibody 20392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,CoIP

Hum Mutat

STAC3 variants cause a congenital myopathy with distinctive dysmorphic features and malignant hyperthermia susceptibility.

Authors - Irina Zaharieva
mouseWB

Structure

Multiple Sequence Variants in STAC3 Affect Interactions with CaV1.1 and Excitation-Contraction Coupling.

Authors - Britany Rufenach
  • KO Validated
mouseWB

Skelet Muscle

The SH3 and cysteine-rich domain 3 (Stac3) gene is important to growth, fiber composition, and calcium release from the sarcoplasmic reticulum in postnatal skeletal muscle.

Authors - Xiaofei Cong
  • KO Validated
humanIF

J Cell Physiol

STAC3 incorporation into skeletal muscle triads occurs independent of the dihydropyridine receptor.

Authors - Marta Campiglio
humanWB

J Gen Physiol

Calcium current modulation by the γ1 subunit depends on alternative splicing of CaV1.1.

Authors - Yousra El Ghaleb
ratWB

J Appl Physiol (1985)

Preconditioning contractions prevent prolonged force depression and Ca2+-dependent proteolysis of STAC3 after damaging eccentric contractions.

Authors - Yuki Ashida
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