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验证数据展示

  • WB analysis using 18004-1-AP

    WB analysis using 18004-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18004-1-AP (ST8SIA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 18004-1-AP

    IHC staining of human liver using 18004-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 18004-1-AP (ST8SIA3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 18004-1-AP

    IHC staining of human liver using 18004-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 18004-1-AP (ST8SIA3 antibody) at dilution of 1:50 (under 40x lens).

ST8SIA3 Polyclonal antibody

ST8SIA3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18004-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

Alpha 2,8 sialyltransferase 8C, Sialyltransferase 8C, Sialytransferase St8Sia III, SIAT8 C, SIAT8C, ST8SIA3, ST8SiaIII

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, mouse liver tissue, Caco-2 cells
Positive IHC detected inhuman liver tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

18004-1-AP targets ST8SIA3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 ST8SIA3 fusion protein Ag12497 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3
别名 Alpha 2,8 sialyltransferase 8C, Sialyltransferase 8C, Sialytransferase St8Sia III, SIAT8 C, SIAT8C, ST8SIA3, ST8SiaIII
计算分子量 380 aa, 44 kDa
观测分子量 44 kDa
GenBank蛋白编号BC074909
基因名称 ST8SIA3
Gene ID (NCBI) 51046
RRIDAB_2239838
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43173
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ST8 α-N-acetyl-neuraminide α-2,8-sialyltransferase (ST8Sia) family catalyzes the synthesis of one or multiple α2,8-linked sialic acid chains according to their acceptor specifificity (PMID:19888308). At least fifive ST8Sia enzymes (ST8SIA1, 2, 3, 4, and 6) have previously been identifified in adult brains (PMID:11530204). The ST8SIA3 is enriched in the striatum. The mouse St8sia3 gene shares 96% amino acid identity with human ST8SIA3, and is mainly expressed in the brain and testis (PMID:31455764).

实验方案

Product Specific Protocols
WB protocol for ST8SIA3 antibody 18004-1-APDownload protocol
IHC protocol for ST8SIA3 antibody 18004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Antiviral Res

Preventing human influenza and coronaviral mono or coinfection by blocking virus-induced sialylation

Authors - Md Ruhul Amin
View Article