验证数据展示
经过测试的应用
Positive WB detected in | COLO 320 cells |
Positive IHC detected in | mouse colon tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human colon cancer tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66209-1-Ig targets ST6GALNAC6 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF-P, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | ST6GALNAC6 fusion protein Ag8358 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 |
别名 | hST6GalNAc VI, RP11 203J24.3, Sialyltransferase 7F, SIAT7 F, SIAT7F, ST6GalNAc VI, ST6GALNAC6, ST6GALNACVI |
计算分子量 | 333aa,38 kDa; 374aa,41 kDa |
观测分子量 | 38-42 kDa |
GenBank蛋白编号 | BC007802 |
基因名称 | ST6GALNAC6 |
Gene ID (NCBI) | 30815 |
RRID | AB_2881600 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q969X2 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ST6GALNAC6 is a sialyltransferase which is responsible for the synthesis of all a-series gangliosides. It has a broad expression pattern. ST6GALNAC6 was reported to be a key enzyme in the synthesis of disialyl monosialyl Lewis (Lea), a representative tumor-associated antigen in cancers of the pancreas and colon.
实验方案
Product Specific Protocols | |
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WB protocol for ST6GALNAC6 antibody 66209-1-Ig | Download protocol |
IHC protocol for ST6GALNAC6 antibody 66209-1-Ig | Download protocol |
IF protocol for ST6GALNAC6 antibody 66209-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |