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验证数据展示

  • IF Staining of human colon cancer using 66209-1-Ig

    IF Staining of human colon cancer using 66209-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using ST6GALNAC6 antibody (66209-1-Ig, Clone: 1A7B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of COLO 320 using 66209-1-Ig

    WB analysis of COLO 320 using 66209-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 66209-1-Ig

    IHC staining of mouse colon using 66209-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 66209-1-Ig

    IHC staining of mouse colon using 66209-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ST6GALNAC6 Monoclonal antibody

ST6GALNAC6 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66209-1-Ig

产品说明书

CloneNo. 1A7B7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6, EC:2.4.99.-, GalNAc alpha-2,6-sialyltransferase VI, hST6GalNAc VI, RP11 203J24.3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inCOLO 320 cells
Positive IHC detected inmouse colon tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66209-1-Ig targets ST6GALNAC6 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag8358

Product name: Recombinant human ST6GALNAC6 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 67-333 aa of BC007802

Sequence: VFHYGSLRGRSRRPVNLKKWSITDGYVPILGNKTLPSRCHQCVIVSSSSHLLGTKLGPEIERAECTIRMNDAPTTGYSADVGNKTTYRVVAHSSVFRVLRRPQEFVNRTPETVFIFWGPPSKMQKPQGSLVRVIQRAGLVFPNMEAYAVSPGRMRQFDDLFRGETGKDREKSHSWLSTGWFTMVIAVELCDHVHVYGMVPPNYCSQRPRLQRMPYHYYEPKGPDECVTYIQNEHSRKGNHHRFITEKRVFSSWAQLYGITFSHPSWT

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6
别名 Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6, EC:2.4.99.-, GalNAc alpha-2,6-sialyltransferase VI, hST6GalNAc VI, RP11 203J24.3
计算分子量333aa,38 kDa; 374aa,41 kDa
观测分子量 38-42 kDa
GenBank蛋白编号BC007802
基因名称 ST6GALNAC6
Gene ID (NCBI) 30815
RRIDAB_2881600
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ969X2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ST6GALNAC6 is a sialyltransferase which is responsible for the synthesis of all a-series gangliosides. It has a broad expression pattern. ST6GALNAC6 was reported to be a key enzyme in the synthesis of disialyl monosialyl Lewis (Lea), a representative tumor-associated antigen in cancers of the pancreas and colon.

实验方案

Product Specific Protocols
IF protocol for ST6GALNAC6 antibody 66209-1-IgDownload protocol
IHC protocol for ST6GALNAC6 antibody 66209-1-IgDownload protocol
WB protocol for ST6GALNAC6 antibody 66209-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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