验证数据展示
经过测试的应用
| Positive IHC detected in | human breast cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
16442-1-AP targets ST6GALNAC5 in IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag9767 Product name: Recombinant human ST6GALNAC5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 28-336 aa of BC001201 Sequence: SLGGQKERPPQQQQQQQQQQQQASATGSSQPAAESSTQQRPGVPAGPRPLDGYLGVADHKPLKMHCRDCALVTSSGHLLHSRQGSQIDQTECVIRMNDAPTRGYGRDVGNRTSLRVIAHSSIQRILRNRHDLLNVSQGTVFIFWGPSSYMRRDGKGQVYNNLHLLSQVLPRLKAFMITRHKMLQFDELFKQETGKDRKISNTWLSTGWFTMTIALELCDRINVYGMVPPDFCRDPNHPSVPYHYYEPFGPDECTMYLSHERGRKGSHHRFITEKRVFKNWARTFNIHFFQPDWKPESLAINHPENKPVF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5 |
| 别名 | Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 5, EC:2.4.99.-, GalNAc alpha-2,6-sialyltransferase V, SIAT7E, SIAT7-E |
| 计算分子量 | 38 kDa |
| GenBank蛋白编号 | BC001201 |
| 基因名称 | ST6GALNAC5 |
| Gene ID (NCBI) | 81849 |
| RRID | AB_10597112 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BVH7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ST6GALNAC5 is a member of the sialyltransferase family, specifically a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to glycoproteins and glycolipids. It plays a critical role in the biosynthesis of sialyl-Tn antigen (sTn), a tumor-associated carbohydrate antigen commonly overexpressed in various adenocarcinomas, including breast, gastric, and colorectal cancers. Aberrant expression of ST6GALNAC5 is closely linked to cancer progression, metastasis, and poor clinical prognosis.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for ST6GALNAC5 antibody 16442-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
