验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue, SH-SY5Y cells, HeLa cells, human adrenal gland tissue, mouse uterus tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12893-1-AP targets ST5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | ST5 fusion protein Ag3403 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | suppression of tumorigenicity 5 |
| 别名 | DENN domain-containing protein 2B, DENND2B, HTS1 |
| 计算分子量 | 1137 aa, 126 kDa |
| 观测分子量 | 82 kDa, 70 kDa |
| GenBank蛋白编号 | BC036655 |
| 基因名称 | ST5 |
| Gene ID (NCBI) | 6764 |
| RRID | AB_10640670 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P78524 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ST5 antibody 12893-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
