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验证数据展示

  • WB analysis using 13154-1-AP (same clone as 13154-1-PBS)

    WB analysis using 13154-1-AP (same clone as 13154-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13154-1-AP (ST3GAL6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13154-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 13154-1-AP (same clone as 13154-1-PBS)

    IHC staining of human liver using 13154-1-AP (same clone as 13154-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13154-1-AP (ST3GAL6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13154-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ST3GAL6 Polyclonal antibody, PBS Only

ST3GAL6 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13154-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13154-1-PBS targets ST3GAL6 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag3782

Product name: Recombinant human ST3GAL6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 25-331 aa of BC023312

Sequence: GTNVYWVAPVEMKRRNKIQPCLSKPAFASLLRFHQFHPFLCAADFRKIASLYGSDKFDLPYGMRTSAEYFRLALSKLQSCDLFDEFDNIPCKKCVVVGNGGVLKNKTLGEKIDSYDVIIRMNNGPVLGHEEEVGRRTTFRLFYPESVFSDPIHNDPNTTVILTAFKPHDLRWLLELLMGDKINTNGFWKKPALNLIYKPYQIRILDPFIIRTAAYELLHFPKVFPKNQKPKHPTTGIIAITLAFYICHEVHLAGFKYNFSDLKSPLHYYGNATMSLMNKNAYHNVTAEQLFLKDIIEKNLVINLTQD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ST3 beta-galactoside alpha-2,3-sialyltransferase 6
别名
计算分子量 331 aa, 38 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC023312
基因名称 ST3GAL6
Gene ID (NCBI) 10402
RRIDAB_10646441
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y274
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ST3GAL6, also named as SIAT10, belongs to the glycosyltransferase 29 family. ST3GAL6 is involved in the synthesis of sialyl-paragloboside, which is a precursor of sialyl-Lewis X determinant. ST3GAL6 has a alpha-2,3-sialyltransferase activity toward Gal-beta1,4-GlcNAc structure on glycoproteins and glycolipids. ST3GAL6 is known to promote cell adhesion and migration of multiple myeloma cells by increasing the synthesis of selectin ligands. ST3GAL6 has 2 isoforms with the molecular mass of 25 and 38 kDa. Sometimes higher molecular weight about 50-60 kDa can also be observed, which may be a glycosylated modified variant of ST3GAL6. (PMID: 31914669, 30220088)

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