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验证数据展示

  • WB analysis using 30120-1-AP (same clone as 30120-1-PBS)

    WB analysis using 30120-1-AP (same clone as 30120-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30120-1-AP (ST3GAL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30120-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 30120-1-AP (same clone as 30120-1-PBS)

    IF Staining of U2OS using 30120-1-AP (same clone as 30120-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ST3GAL5 antibody (30120-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30120-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 30120-1-AP (same clone as 30120-1-PBS)

    IF Staining of U2OS using 30120-1-AP (same clone as 30120-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ST3GAL5 antibody (30120-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30120-1-PBS in a different storage buffer formulation.

ST3GAL5 Polyclonal antibody, PBS Only

ST3GAL5 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 30120-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

30120-1-PBS targets ST3GAL5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32461

Product name: Recombinant human ST3GAL5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 230-418 aa of BC065936

Sequence: YSEHVGNKTTIRMTYPEGAPLSDLEYYSNDLFVAVLFKSVDFNWLQAMVKKETLPFWVRLFFWKQVAEKIPLQPKHFRILNPVIIKETAFDILQYSEPQSRFWGRDKNVPTIGVIAVVLATHLCDEVSLAGFGYDLNQPRTPLHYFDSQCMAAMNFQTMHNVTTETKFLLKLVKEGVVKDLSGGIDREF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ST3 beta-galactoside alpha-2,3-sialyltransferase 5
别名
计算分子量 48 kDa
观测分子量 70 kDa
GenBank蛋白编号BC065936
基因名称 ST3GAL5
Gene ID (NCBI) 8869
RRIDAB_3086237
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UNP4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ST3GAL5 is also named as SIAT9, GM3 synthase, ST3Gal V, Sialyltransferase 9 and belongs to the glycosyltransferase 29 family. ST3GAL5 transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc to the non-reducing terminal galactose of glycosphingolipids forming gangliosides (PMID:9822625, PMID:16934889). SIAT9 is mainly involved in the biosynthesis of ganglioside GM3 but can also use different glycolipids as substrate acceptors such as D-galactosylceramide (GalCer), asialo-GM2 (GA2) and asialo-GM1 (GA1) (PMID:16934889). ST3GAL5 is high expression in brain, skeletal muscle, placenta, and testis. And mRNA of ST3GAL5 is widely distributed in human brain, but slightly elevated expression was observed in the cerebral cortex, temporal lobe, and putamen (PMID:9822625). This antibody recognizes the glycosylated form of ST3GAL5 (PMID: 34943806, PMID: 26458842).

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