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ST13 Polyclonal antibody

ST13 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 26581-1-AP

Print datasheet

Synonyms

AAG2, FAM10A1, FAM10A4, HIP, HOP, Hsc70 interacting protein, HSPABP, HSPABP1, P48, PRO0786, Protein FAM10A1, Putative tumor suppressor ST13, SNC6, ST13

验证数据展示

  • WB analysis of HEK-293 using 26581-1-AP

    WB analysis of HEK-293 using 26581-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 26581-1-AP (ST13 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 26581-1-AP

    IP experiment of HEK-293 using 26581-1-AP

    IP result of anti-ST13 (IP:26581-1-AP, 4ug; Detection:26581-1-AP 1:2000) with HEK-293 cells lysate 1800ug.

  • IHC staining of human colon cancer using 26581-1-AP

    IHC staining of human colon cancer using 26581-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26581-1-AP (ST13 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ST13 antibody included
  • IHC staining of human colon cancer using 26581-1-AP

    IHC staining of human colon cancer using 26581-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26581-1-AP (ST13 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ST13 antibody included

经过测试的应用

Positive WB detected inHEK-293 cells, mouse testis tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ST13 Ready-To-Use IHC Kit. ST13 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26581-1-AP is ST13 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen ST13 fusion protein Ag24779 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein)
Synonyms AAG2, FAM10A1, FAM10A4, HIP, HOP, Hsc70 interacting protein, HSPABP, HSPABP1, P48, PRO0786, Protein FAM10A1, Putative tumor suppressor ST13, SNC6, ST13
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC139724
Gene Symbol ST13
Gene ID (NCBI) 6767
RRIDAB_2880560
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50502
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ST13 antibody 26581-1-APDownload protocol
IHC protocol for ST13 antibody 26581-1-APDownload protocol
IP protocol for ST13 antibody 26581-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Sci Rep

Novel candidate factors predicting the effect of S-1 adjuvant chemotherapy of pancreatic cancer.

Authors - Katsutaka Mitachi
View Article
mouseWB

Cancer Res

N-myc-Mediated Translation Control Is a Therapeutic Vulnerability in Medulloblastoma

Authors - Duygu Kuzuoglu-Ozturk
View Article