验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IF | See 3 publications below |
产品信息
11797-1-AP targets NA14 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2367 Product name: Recombinant human NA14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-119 aa of BC000864 Sequence: MTQQGAALQNYNNELVKCIEELCQKREELCRQIQEEEDEKQRLQNEVRQLTEKLARVNENLARKIASRNEFDRTIAETEAAYLKILESSQTLLSVLKREAGNLTKATAPDQKSSGGRDS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Sjogren syndrome nuclear autoantigen 1 |
| 别名 | N14, NA 14, NA14, Nuclear autoantigen of 14 kDa, SSNA1 |
| 计算分子量 | 119 aa, 14 kDa |
| 观测分子量 | 14 kDa |
| GenBank蛋白编号 | BC000864 |
| 基因名称 | SSNA1 |
| Gene ID (NCBI) | 8636 |
| RRID | AB_2195497 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43805 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SSNA1, also named as NA14 and DIP13, may be an important adaptor to target spastin activity at the centrosome. SSNA1 is a 14 kDa protetin which locates the basal bodies and flagella of human sperm cells and the centrosome of cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NA14 antibody 11797-1-AP | Download protocol |
| IHC protocol for NA14 antibody 11797-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
MBio The cellular protein La functions in enhancement of virus release through lipid rafts facilitated by murine leukemia virus glycosylated Gag. | ||
Mol Biol Cell Functional characterization of putative cilia genes by high-content analysis. | ||
PLoS One Spastin-Interacting Protein NA14/SSNA1 Functions in Cytokinesis and Axon Development. | ||
Biosci Trends Identification of MIWI-associated Poly(A) RNAs by immunoprecipitation with an anti-MIWI monoclonal antibody. |
