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SSH2 Polyclonal antibody

SSH2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20674-1-AP

Print datasheet

Synonyms

hSSH 2L, KIAA1725, SSH 2, SSH 2L, SSH like protein 2, SSH2, SSH2L

验证数据展示

  • WB analysis using 20674-1-AP

    WB analysis using 20674-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20674-1-AP (SSH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 20674-1-AP

    IHC staining of human liver using 20674-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20674-1-AP (SSH2 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 20674-1-AP

    IHC staining of human liver using 20674-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20674-1-AP (SSH2 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, MG-63 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20674-1-AP targets SSH2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name slingshot homolog 2 (Drosophila)
Synonyms hSSH 2L, KIAA1725, SSH 2, SSH 2L, SSH like protein 2, SSH2, SSH2L
Calculated Molecular Weight 158 kDa
Observed Molecular Weight 45-51 kDa
GenBank Accession NumberNM_033389
Gene Symbol SSH2
Gene ID (NCBI) 85464
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ76I76
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SSH2 antibody 20674-1-APDownload protocol
IHC protocol for SSH2 antibody 20674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Adh Migr

The modulation of actin dynamics via atypical protein kinase-C activated Cofilin regulates metastasis of colorectal cancer cells.

Authors - S M Anisul Islam
View Article