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  • Featured Product
  • KD/KO Validated

SSBP1 Polyclonal antibody

SSBP1 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 12212-1-AP

Print datasheet

Synonyms

Mt SSB, MtSSB, PWP1 interacting protein 17, SSBP, SSBP1

验证数据展示

  • WB analysis of A549 using 12212-1-AP

    WB analysis of A549 using 12212-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IF Staining of A375 using 12212-1-AP

    IF Staining of A375 using 12212-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using 12212-1-AP (SSBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 12212-1-AP

    WB analysis using 12212-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12212-1-AP (SSBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, A375 cells, mouse pancreas tissue, rat pancreas tissue, Caco-2 cells
Positive IF detected inA375 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12212-1-AP targets SSBP1 in WB, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB, IF, IHC, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen SSBP1 fusion protein Ag2860 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name single-stranded DNA binding protein 1
Synonyms Mt SSB, MtSSB, PWP1 interacting protein 17, SSBP, SSBP1
Calculated Molecular Weight 148 aa, 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC000895
Gene Symbol SSBP1
Gene ID (NCBI) 6742
RRIDAB_2195320
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ04837
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SSBP1 is a housekeeping gene involved in mitochondrial biogenesis. it binds preferentially and cooperatively to ss-DNA in the maintainance of genome stability. SSBP1 acted as a homotetramer to stabilize the displaced single strand of the normal and expanded displacement loop (D loop) during mtDNA replication, thus preventing formation of secondary single-stranded DNA structures, which could stop the gamma-DNA polymerase

实验方案

Product Specific Protocols
WB protocol for SSBP1 antibody 12212-1-APDownload protocol
IF protocol for SSBP1 antibody 12212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

MOF Acetyl Transferase Regulates Transcription and Respiration in Mitochondria.

Authors - Aindrila Chatterjee
View Article
humanWB

Nat Cell Biol

Mitochondrial RNA granules are fluid condensates positioned by membrane dynamics.

Authors - Timo Rey
View Article
humanWB

Mol Cell

Single-Molecule Analysis of mtDNA Replication Uncovers the Basis of the Common Deletion.

Authors - Aaron F Phillips
View Article

Sci Adv

The mitochondrial single-stranded DNA binding protein is essential for initiation of mtDNA replication.

Authors - Min Jiang
  • KO Validated
View Article
humanIHC

Nucleic Acids Res

Top3α is the replicative topoisomerase in mitochondrial DNA replication.

Authors - Anu Hangas
View Article
humanWB

Brain

Heterozygous SSBP1 start loss mutation co-segregates with hearing loss and the m.1555A>G mtDNA variant in a large multigenerational family.

Authors - Peter J Kullar
View Article