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验证数据展示

  • WB analysis using 22185-1-AP

    WB analysis using 22185-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22185-1-AP (SS18 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 22185-1-AP

    IHC staining of mouse colon using 22185-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22185-1-AP (SS18 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 22185-1-AP

    IHC staining of mouse colon using 22185-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22185-1-AP (SS18 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SS18 Polyclonal antibody

SS18 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22185-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Protein SSXT, Protein SYT, SS18, SSXT, SYT, SYT SSX1, SYT SSX2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22185-1-AP targets SS18 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag16743

Product name: Recombinant human SS18 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 101-281 aa of BC096222

Sequence: SHNMPSDGMVGGGPPAPHMQNQMNGQMPGPNHMPMQGPGPNQLNMTNSSMNMPSSSHGSMGGYNHSVPSSQSMPVQNQMTMSQGQPMGNYGPRPNMSMQPNQGPMMHQQPPSQQYNMPQGGGQHYQGQQPPMGMMGQVNQGNHMMGQRQIPPYRPPQQGPPQQYSGQEDYYGDQYSHGGQG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synovial sarcoma translocation, chromosome 18
别名 Protein SSXT, Protein SYT, SS18, SSXT, SYT, SYT SSX1, SYT SSX2
计算分子量 418 aa, 46 kDa
观测分子量50-60 kDa
GenBank蛋白编号BC096222
基因名称 SS18
Gene ID (NCBI) 6760
RRIDAB_3669390
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15532
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for SS18 antibody 22185-1-APDownload protocol
WB protocol for SS18 antibody 22185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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