验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, DU 145 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67349-1-Ig targets SRY in WB, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human |
| 免疫原 | SRY fusion protein Ag12610 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | sex determining region Y |
| 别名 | Testis-determining factor, Sex-determining region Y protein, sex determining region Y, 2E9A4 |
| 计算分子量 | 204 aa, 24 kDa |
| 观测分子量 | 25-30 kDa |
| GenBank蛋白编号 | BC074924 |
| 基因名称 | SRY |
| Gene ID (NCBI) | 6736 |
| RRID | AB_2882607 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q05066 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SRY (sex-determining region Y protein) is a tran-scriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SRY antibody 67349-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
