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SRX1 Polyclonal antibody

SRX1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29849-1-AP

Print datasheet

Synonyms

SRX, SRX1, SRXN1, Sulfiredoxin 1

验证数据展示

  • WB analysis using 29849-1-AP

    WB analysis using 29849-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29849-1-AP (SRX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 29849-1-AP

    IHC staining of human colon cancer using 29849-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29849-1-AP (SRX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, PC-12 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29849-1-AP targets SRX1 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, rat
Immunogen SRX1 fusion protein Ag31537 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sulfiredoxin 1 homolog (S. cerevisiae)
Synonyms SRX, SRX1, SRXN1, Sulfiredoxin 1
Calculated Molecular Weight14KD
Observed Molecular Weight13-16 kDa
GenBank Accession NumberNM_080725
Gene Symbol SRXN1
Gene ID (NCBI) 140809
RRIDAB_2918350
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYN0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SRX1 antibody 29849-1-APDownload protocol
IHC protocol for SRX1 antibody 29849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols