• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis using 85926-2-RR

    WB analysis using 85926-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85926-2-RR (SRPRB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 85926-2-RR

    IHC staining of mouse liver using 85926-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85926-2-RR (SRPRB antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 85926-2-RR

    IHC staining of mouse brain using 85926-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85926-2-RR (SRPRB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 85926-2-RR

    IF Staining of HepG2 using 85926-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SRPRB antibody (85926-2-RR, Clone: 242807E8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85926-2-RR

    Affinity and Kinetic Characterization of 85926-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85926-2-RR against Human SRPRB were performed. The affinity constant is 82.7 pM.

SRPRB Recombinant monoclonal antibody

SRPRB Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85926-2-RR

产品说明书

CloneNo. 242807E8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Protein APMCF1, PSEC0230, Signal recognition particle receptor subunit beta, SR-beta

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, L02 cells, MCF-7 cells, COLO 320 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse brain tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85926-2-RR targets SRPRB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6215

Product name: Recombinant human SRPRB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-271 aa of BC065299

Sequence: MASADSRRVADGGGAGGTFQPYLDTLRQELQQTDPTLLSVVVAVLAVLLTLVFWKLIRSRRSSQRAVLLVGLCDSGKTLLFVRLLTGLYRDTQTSITDSCAVYRVNNNRGNSLTLIDLPGHESLRLQFLERFKSSARAIVFVVDSAAFQREVKDVAEFLYQVLIDSMGLKNTPSFLIACNKQDIAMAKSAKLIQQQLEKELNTLRVTRSAAPSTLDSSSTAPAQLGKKGKEFEFSQLPLKVEFLECSAKGGRGDVGSADIQDLEKWLAKIA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 signal recognition particle receptor, B subunit
别名 Protein APMCF1, PSEC0230, Signal recognition particle receptor subunit beta, SR-beta
计算分子量 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC065299
基因名称 SRPRB
Gene ID (NCBI) 58477
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y5M8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for SRPRB antibody 85926-2-RRDownload protocol
IHC protocol for SRPRB antibody 85926-2-RRDownload protocol
WB protocol for SRPRB antibody 85926-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
||
New chat Able
正在加载，请稍候...