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验证数据展示

  • WB analysis using 85613-5-RR

    WB analysis using 85613-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85613-5-RR (SRPK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85613-5-RR

    Affinity and Kinetic Characterization of 85613-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85613-5-RR against Human SRPK2 were performed. The affinity constant is 4.16 nM.

SRPK2 Recombinant antibody

SRPK2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85613-5-RR

产品说明书

CloneNo. 242935B1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

EC:2.7.11.1, Serine/arginine-rich protein-specific kinase 2, SFRS protein kinase 2, SFRSK2, SR protein specific kinase 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy SRPK2 Ready-To-Use IHC Kit. SRPK2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85613-5-RR targets SRPK2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 SRPK2 fusion protein Ag17885 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SFRS protein kinase 2
别名 EC:2.7.11.1, Serine/arginine-rich protein-specific kinase 2, SFRS protein kinase 2, SFRSK2, SR protein specific kinase 2
计算分子量 688 aa, 78 kDa
观测分子量 105-110 kDa
GenBank蛋白编号BC035214
基因名称 SRPK2
Gene ID (NCBI) 6733
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP78362
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SRPK2, also named as serine/arginine protein kinase 2 and SFRSK2, belongs to SR protein-specific kinase family which phosphorylate the serine/arginine domain containing proteins in the nuclear speckles to mediate pre-mRNA splicing (PMID: 22854045). In addition to its major role as kinase for the phosphorylation of SR proteins, SRPK2 is involved in cell cycle regulation and cell apoptosis (PMID: 19592491). SRPK2 promotes the proliferation and migration of the colon cancer cells. It has been reported to be regulated in several cancer types (PMID: 27041240). It has 2 isoforms with the calculated molecular mass of 77-79 kDa, and the apparent molecular mass of 105-115 kDa due to posttranslational modification (PMID: 12134018, 16122776).

实验方案

Product Specific Protocols
WB protocol for SRPK2 antibody 85613-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols