验证数据展示
经过测试的应用
| Positive WB detected in | HSC-T6 cells, HeLa cells, HEK-293 cells, ROS1728 cells, NIH/3T3 cells, 4T1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
66068-1-Ig targets SRP9 in WB, ELISA applications and shows reactivity with human, rat, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, rat, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag17153 Product name: Recombinant human SRP9 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-86 aa of BC015094 Sequence: MPQYQTWEEFSRAAEKLYLADPMKARVVLKYRHSDGNLCVKVTDDLVCLVYKTDQAQDVKKIEKFHSQLMRLMVAKEARNVTMETE 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | signal recognition particle 9kDa |
| 别名 | ALURBP, SRP9 |
| 计算分子量 | 10 kDa |
| 观测分子量 | 10 kDa |
| GenBank蛋白编号 | BC015094 |
| 基因名称 | SRP9 |
| Gene ID (NCBI) | 6726 |
| RRID | AB_11042618 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P49458 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Signal recognition particle 9(SRP9) is component of the signal recognition particle (SRP), which is a ribonucleoprotein complex that mediates the targeting of proteins to the rough endoplasmic reticulum (ER). SRP9 form a heterodimer with SRP14, which involves in arrest of the elongation of the nescent chains during targeting to ensure efficient translocation of the preprotein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SRP9 antibody 66068-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
bioRxiv Variable patterns of retrotransposition in different HeLa strains provide mechanistic insights into SINE RNA mobilization processes | ||
Nucleic Acids Res Variable patterns of retrotransposition in different HeLa strains provide mechanistic insights into SINE RNA mobilization processes |
