验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, NIH/3T3 cells |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human breast cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
11729-1-AP targets SRP54 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | SRP54 fusion protein Ag2327 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | signal recognition particle 54kDa |
| 别名 | Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4 |
| 计算分子量 | 54 kDa |
| 观测分子量 | 54 kDa |
| GenBank蛋白编号 | BC003389 |
| 基因名称 | SRP54 |
| Gene ID (NCBI) | 6729 |
| RRID | AB_2194724 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P61011 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SRP54 antibody 11729-1-AP | Download protocol |
| IHC protocol for SRP54 antibody 11729-1-AP | Download protocol |
| IF protocol for SRP54 antibody 11729-1-AP | Download protocol |
| IP protocol for SRP54 antibody 11729-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Estrogen-Related Hormones Induce Apoptosis by Stabilizing Schlafen-12 Protein Turnover. | ||
Biochem Biophys Res Commun Polyglutamine expansion disturbs the endoplasmic reticulum formation, leading to caspase-7 activation through Bax. | ||
Biochem Biophys Res Commun Expanded polyglutamine embedded in the endoplasmic reticulum causes membrane distortion and coincides with Bax insertion. | ||
iScience Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges |
