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验证数据展示

  • IP experiment of HL-60 using 26447-1-AP

    IP experiment of HL-60 using 26447-1-AP

    IP result of anti-SRMS (IP:26447-1-AP, 4ug; Detection:26447-1-AP 1:300) with HL-60 cells lysate 2800ug.

  • IHC staining of human breast cancer using 26447-1-AP

    IHC staining of human breast cancer using 26447-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26447-1-AP (SRMS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 26447-1-AP

    IHC staining of human breast cancer using 26447-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26447-1-AP (SRMS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SRMS Polyclonal antibody

SRMS Polyclonal Antibody for IP, IHC, ELISA
Cat No. 26447-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IP, IHC, ELISA

别名

C20orf148, SRM, SRMS, Tyrosine protein kinase Srms

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inHL-60 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26447-1-AP targets SRMS in IP, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IP, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag24062

Product name: Recombinant human SRMS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 418-488 aa of BC153198

Sequence: EVFTYGQCPYEGMTNHETLQQIMRGYRLPRPAACPAEVYVLMLECWRSSPEERPSFATLREKLHAIHRCHP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristylation sites
别名 C20orf148, SRM, SRMS, Tyrosine protein kinase Srms
计算分子量 488 aa, 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC153198
基因名称 SRMS
Gene ID (NCBI) 6725
RRIDAB_2880516
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H3Y6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for SRMS antibody 26447-1-APDownload protocol
IP protocol for SRMS antibody 26447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols