Spermidine synthase Monoclonal antibody
Spermidine synthase Monoclonal Antibody for IF/ICC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
IF/ICC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2F4E10
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells |
Positive IF detected in | RAW 264.7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68548-1-Ig targets Spermidine synthase in WB, IF, ELISA applications and shows reactivity with Human samples.
Tested Applications | IF/ICC, WB, ELISA |
Tested Reactivity | Human |
Immunogen | Spermidine synthase fusion protein Ag33919 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | spermidine synthase |
Synonyms | PAPT, SPDSY, spermidine synthase, SPS1, SRM, SRML1 |
Calculated Molecular Weight | 302 aa, 34 kDa |
Observed Molecular Weight | 34 kDa |
GenBank Accession Number | BC000309 |
Gene Symbol | SRM |
Gene ID (NCBI) | 6723 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P19623 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Spermidine synthase is an enzyme that catalyzes the transfer of the propylamine group from S-adenosylmethioninamine to putrescine in the biosynthesis of spermidine. The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthases catalyze the production of the linear triamine, spermidine, from putrescine. They utilize decarboxylated S-adenosylmethionine (dc-SAM), a universal cofactor of aminopropyltransferases, as a donor of the aminopropyl moiety (PMID:31134111).
实验方案
Product Specific Protocols | |
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WB protocol for Spermidine synthase antibody 68548-1-Ig | Download protocol |
IF protocol for Spermidine synthase antibody 68548-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |