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SRGAP3 Polyclonal antibody

SRGAP3 Polyclonal Antibody for IF-P, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse , Rat

Applications

IF-P, WB, ELISA

Conjugate

Unconjugated

Cat No : 29624-1-AP

Print datasheet

Synonyms

ARHGAP14, KIAA0411, KIAA1156, MEGAP, Mental disorder associated GAP, SRGAP2, SRGAP3, WRP

验证数据展示

  • WB analysis using 29624-1-AP

    WB analysis using 29624-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29624-1-AP (SRGAP3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 29624-1-AP

    IF Staining of mouse brain using 29624-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SRGAP3 antibody (29624-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29624-1-AP targets SRGAP3 in WB, IF, ELISA applications and shows reactivity with Human, Mouse , Rat samples.

Tested Applications IF-P, WB, ELISA
Tested Reactivity Human, Mouse , Rat
Immunogen SRGAP3 fusion protein Ag31196 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SLIT-ROBO Rho GTPase activating protein 3
Synonyms ARHGAP14, KIAA0411, KIAA1156, MEGAP, Mental disorder associated GAP, SRGAP2, SRGAP3, WRP
Calculated Molecular Weight 124 kDa
Observed Molecular Weight125 kDa
GenBank Accession NumberNM_014850
Gene Symbol SRGAP3
Gene ID (NCBI) 9901
RRIDAB_2918334
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43295
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

SRGAP3 (SLIT-ROBO Rho GTPase Activating Protein 3), also named MEGAP and WRP, is a member of the Slit-Robo sub-family of Rho GTPase-activating proteins (Rho GAPs), controls actin and microtubule dynamics through negative regulation of Rac(PMID: 23108406). Diseases associated with SRGAP3 include Pilomyxoid Astrocytoma and Avoidant Personality Disorder. SRGAP3 is highly expressed in fetal and adult brain, including the cortex and the hippocampus.

实验方案

Product Specific Protocols
WB protocol for SRGAP3 antibody 29624-1-APDownload protocol
IF protocol for SRGAP3 antibody 29624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols