验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse brain tissue, Apoptosised HeLa cells, mouse kidney tissue, HEK-293 cells, SH-SY5Y cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human liver cancer tissue, mouse testis tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SRGAP1 Ready-To-Use IHC Kit. SRGAP1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13252-1-AP targets SRGAP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag4104 Product name: Recombinant human SRGAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC029919 Sequence: MSTPSRFKKDKEIIAEYESQVKEIRAQLVEQQKCLEQQTEMRVQLLQDLQDFFRKKAEIETEYSRNLEKLAERFMAKTRSTKDHQQYKKDQNLLSPVNCWYLLLNQVRRESKDHATLSDIYLNNVIMRFMQISEDSTRMFKKSKEIAFQLHEDLMKVLNELYTVMKTYHMYHAESISAESKLKEAEKQEEKQIGRSGDPVFHIRLEERHQRRSSVKKIEKMKEKRQAKYSENKLKSIKARNEYLLTLEATNASVFKYYIHDLSDLIDCCDLGYHASLNRALRTYLSAEYNLETSRHEGLD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SLIT-ROBO Rho GTPase activating protein 1 |
| 别名 | ARHGAP13, KIAA1304, SRGAP1 |
| 计算分子量 | 1085 aa, 124 kDa |
| 观测分子量 | 124 kDa |
| GenBank蛋白编号 | BC029919 |
| 基因名称 | SRGAP1 |
| Gene ID (NCBI) | 57522 |
| RRID | AB_2270916 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z6B7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SRGAP1 antibody 13252-1-AP | Download protocol |
| IP protocol for SRGAP1 antibody 13252-1-AP | Download protocol |
| WB protocol for SRGAP1 antibody 13252-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Am Soc Nephrol SRGAP1 Controls Small Rho GTPases To Regulate Podocyte Foot Process Maintenance. | ||
J Exp Clin Cancer Res srGAP1 mediates the migration inhibition effect of Slit2-Robo1 in colorectal cancer. | ||
Cell Rep A proliferative to invasive switch is mediated by srGAP1 downregulation through the activation of TGF-β2 signaling |
