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验证数据展示

  • IHC staining of human pancreas cancer using 12792-1-AP

    IHC staining of human pancreas cancer using 12792-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12792-1-AP (SRFBP1 antibody) at dilution of 1:100 (under 10x lens).

  • WB analysis of mouse heart using 12792-1-AP

    WB analysis of mouse heart using 12792-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12792-1-AP (SRFBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SRFBP1 Polyclonal antibody

SRFBP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12792-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

BUD22, P49, p49/STRAP, Rlb1, SRFBP1, STRAP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, HeLa cells, HepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12792-1-AP targets SRFBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SRFBP1 fusion protein Ag3517 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serum response factor binding protein 1
别名 BUD22, P49, p49/STRAP, Rlb1, SRFBP1, STRAP
计算分子量 429 aa, 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC031222
基因名称 SRFBP1
Gene ID (NCBI) 153443
RRIDAB_2877882
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NEF9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SRFBP1, also named as P49, p49/STRAP and STRAP, is abundantly expressed in heart and skeletal muscle, and at much lower levels in brain and lung. It may be involved in regulating transcriptional activation of cardiac genes during the aging process. SRFBP1 may play a role in biosynthesis and/or processing of SLC2A4 in adipose cells. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human SRFBP1.

实验方案

Product Specific Protocols
WB protocol for SRFBP1 antibody 12792-1-APDownload protocol
IHC protocol for SRFBP1 antibody 12792-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols