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验证数据展示

  • IF Staining of HeLa using 83403-1-RR (same clone as 83403-1-PBS)

    IF Staining of HeLa using 83403-1-RR (same clone as 83403-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SREBF2 antibody (83403-1-RR, Clone: 240451A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83403-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83403-1-RR (same clone as 83403-1-PBS)

    FC experiment of HeLa using 83403-1-RR (same clone as 83403-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SREBF2 Recombinant antibody (83403-1-RR, Clone:240451A12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83403-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83403-1-RR

    Affinity and Kinetic Characterization of 83403-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83403-1-RR against Human SREBF2 were performed. The affinity constant is 0.536 nM.

SREBF2 Recombinant monoclonal antibody, PBS Only

SREBF2 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83403-1-PBS

产品说明书

CloneNo. 240451A12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

240451A12, bHLHd2, Class D basic helix-loop-helix protein 2, Processed sterol regulatory element-binding protein 2, SREBF 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SREBF2 Ready-To-Use IHC Kit. SREBF2 primary antibody included.

产品信息

83403-1-PBS targets SREBF2 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag28205

Product name: Recombinant human SREBF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 375-479 aa of BC056158

Sequence: DYIKYLQQVNHKLRQENMVLKLANQKNKLLKGIDLGSLVDNEVDLKIEDFNQNVLLMSPPASDSGSQAGFSPYSIDSEPGSPLLDDAKVKDEPDSPPVALGMVDR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sterol regulatory element binding transcription factor 2
别名 240451A12, bHLHd2, Class D basic helix-loop-helix protein 2, Processed sterol regulatory element-binding protein 2, SREBF 2
计算分子量 124 kDa
GenBank蛋白编号BC056158
基因名称 SREBF2
Gene ID (NCBI) 6721
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ12772
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis.

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