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验证数据展示

  • IHC staining of human kidney using 66875-1-Ig (same clone as 66875-1-PBS)

    IHC staining of human kidney using 66875-1-Ig (same clone as 66875-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66875-1-Ig (SREBF1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SREBF1 antibody included
  • IF Staining of HepG2 using 66875-1-Ig (same clone as 66875-1-PBS)

    IF Staining of HepG2 using 66875-1-Ig (same clone as 66875-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SREBF1 antibody (66875-1-Ig, Clone: 1B6G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). CL594-Phalloidin stains the F-actin (red). DAPI (blue). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66875-1-Ig (same clone as 66875-1-PBS)

    WB analysis of HeLa using 66875-1-Ig (same clone as 66875-1-PBS)

    WB result of SREBF1 antibody (66875-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SREBF1 transfected HeLa cells. This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

  • WB analysis using 66875-1-Ig (same clone as 66875-1-PBS)

    WB analysis using 66875-1-Ig (same clone as 66875-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66875-1-Ig (SREBF1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

  • WB analysis using 66875-1-Ig (same clone as 66875-1-PBS)

    WB analysis using 66875-1-Ig (same clone as 66875-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66875-1-Ig (SREBF1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66875-1-Ig (same clone as 66875-1-PBS)

    IF Staining of HepG2 using 66875-1-Ig (same clone as 66875-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SREBF1 antibody (66875-1-Ig, Clone: 1B6G5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66875-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SREBF1 Monoclonal antibody, PBS Only

SREBF1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66875-1-PBS

产品说明书

CloneNo. 1B6G5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

SREBP 1, SREBF 1, Processed sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1, bHLHd1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SREBF1 Ready-To-Use IHC Kit. SREBF1 primary antibody included.

产品信息

66875-1-PBS targets SREBF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SREBF1 fusion protein Ag5484 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 sterol regulatory element binding transcription factor 1
别名 SREBP 1, SREBF 1, Processed sterol regulatory element-binding protein 1, Class D basic helix-loop-helix protein 1, bHLHd1
计算分子量 1177 aa, 125 kDa
观测分子量 125 kDa
GenBank蛋白编号BC063281
基因名称 SREBF1
Gene ID (NCBI) 6720
RRIDAB_2882209
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP36956
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SREBF1, also named as BHLHD1 and SREBP1, contains one basic helix-loop-helix (bHLH) domain and belongs to the SREBP family. It is a transcriptional activator required for lipid homeostasis. The SREBPs are synthesized as precursors anchored to endoplasmic reticulum (ER) membranes and complexed with SCAP. When the cellular cholesterol level is low, SREBP-SCAP complexes move to the Golgi apparatus, where SREBPs undergo a two-step proteolytic processing, leading to the release of the mature form, an N-terminal fragment, i.e, basic helix-loop-helix leucine zipper transcription factor. These factors enter the nucleus where they bind to sterol regulatory elements (SRE) in the promoter regions of a number of genes whose products mediate the synthesis of cholesterol and fatty acids. [PMID: 21698267]. This antibody can recognize the 125kd precursor form and the 68kd mature form of human SREBF1.