SRD5A1 Polyclonal antibody

SRD5A1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26001-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA and More (1)

S5AR 1, SR type 1, SRD5A1, Steroid 5 alpha reductase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse testis tissue, DU 145 cells
Positive IHC detected inhuman colon cancer tissue, human prostate cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26001-1-AP targets SRD5A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 SRD5A1 fusion protein Ag23351 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 steroid-5-alpha-reductase, alpha polypeptide 1 (3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1)
别名 S5AR 1, SR type 1, SRD5A1, Steroid 5 alpha reductase 1
观测分子量 29 kDa
GenBank蛋白编号BC007033
基因名称 SRD5A1
Gene ID (NCBI) 6715
RRIDAB_2880328
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP18405
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Steroid 5-alpha-reductase (SRD5A1) catalyzes the conversion of 17β-Hydroxyandrost-4-en-3-one into the more potent androgen, DHT. SRD5A1 is a minor component of the reductase activity in prostate although the gene was originally cloned from prostate. On the other hand, SRD5A1 appears to be the predominant isozyme of steroid 5-alpha-reductase in the scalp and elsewhere in the skin.

实验方案

Product Specific Protocols
WB protocol for SRD5A1 antibody 26001-1-APDownload protocol
IHC protocol for SRD5A1 antibody 26001-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Sci Rep

Pao Pereira Extract Attenuates Testosterone-Induced Benign Prostatic Hyperplasia in Rats by inhibiting 5α-Reductase.

Authors - Jiakuan Liu
humanWB

Cancer Med

Integrated whole-exome and bulk transcriptome sequencing delineates the dynamic evolution from preneoplasia to invasive lung adenocarcinoma featured with ground-glass nodules

Authors - Dong Zhou
mouseWB

J Steroid Biochem Mol Biol

Syce1 and Syce3 regulate testosterone and dihydrotestosterone synthesis via steroidogenic pathways in mouse Sertoli and Leydig cells.

Authors - Qi Wang
ratWB

J Integr Med

Rauwolfia vomitoria extract suppresses benign prostatic hyperplasia by reducing expression of androgen receptor and 5α-reductase in a rat model.

Authors - Tian Fang
mouseIF

Biomedicines

Mixed-Culture Propagation of Uterine-Tissue-Resident Macrophages and Their Expression Properties of Steroidogenic Molecules

Authors - Kazushige Ogawa
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