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NCOA1/SRC-1 Polyclonal antibody

NCOA1/SRC-1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 21952-1-AP

Print datasheet

Synonyms

bHLHe42, bHLHe74, F SRC 1, KAT13A, NCoA 1, NCOA1, nuclear receptor coactivator 1, Protein Hin 2, RIP160, SRC 1, SRC1, Steroid receptor coactivator 1

验证数据展示

  • WB analysis using 21952-1-AP

    WB analysis using 21952-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21952-1-AP (NCOA1/SRC-1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJurkat cells, Raji cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21952-1-AP targets NCOA1/SRC-1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA
Tested Reactivity human
Immunogen NCOA1/SRC-1 fusion protein Ag16603 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear receptor coactivator 1
Synonyms bHLHe42, bHLHe74, F SRC 1, KAT13A, NCoA 1, NCOA1, nuclear receptor coactivator 1, Protein Hin 2, RIP160, SRC 1, SRC1, Steroid receptor coactivator 1
Calculated Molecular Weight 1441 aa, 157 kDa
Observed Molecular Weight 140-160 kDa
GenBank Accession NumberBC111533
Gene Symbol NCOA1
Gene ID (NCBI) 8648
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ15788
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear receptor coactivator 1, also named SRC-1, directly binds nuclear receptors and stimulates transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR, and ER), retinoids (RXRs), thyroid hormone (TRs), and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B, and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via the remodeling of chromatin, and possibly act by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.

实验方案

Product Specific Protocols
WB protocol for NCOA1/SRC-1 antibody 21952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols