验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, MCF-7 cells |
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy SRA1 Ready-To-Use IHC Kit. SRA1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
18699-1-AP targets SRA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | steroid receptor RNA activator 1 |
| 别名 | pp7684, SRA, SRA1, SRAP, STRAA1 |
| 计算分子量 | 26 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC156760 |
| 基因名称 | SRA1 |
| Gene ID (NCBI) | 10011 |
| RRID | AB_2270887 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9HD15 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Steroid receptor RNA activator 1 (SRA1), also known as seroid receptor RNA activator protein (SRAP) or SRA, is involved in modulating the activity of multiple transcription factors including the estrogen receptor (ER) (PMID: 20398657). It may play a role in tumorigenesis (PMID: 16152589). The SRA1 gene encodes both SRA1 protein and a non-coding functional RNA that functions as part of a ribonucleoprotein complex activating steroid receptor induced transcription.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for SRA1 antibody 18699-1-AP | Download protocol |
| WB protocol for SRA1 antibody 18699-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Atherosclerosis Flagellar hook protein FlgE promotes macrophage activation and atherosclerosis by targeting ATP5B | ||
Transl Neurodegener Nanotechnology for microglial targeting and inhibition of neuroinflammation underlying Alzheimer's pathology |
