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验证数据展示

  • WB analysis of A549 using 67206-1-Ig (same clone as 67206-1-PBS)

    WB analysis of A549 using 67206-1-Ig (same clone as 67206-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 67206-1-Ig (SQLE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 67206-1-Ig (same clone as 67206-1-PBS)

    IF Staining of HEK-293 using 67206-1-Ig (same clone as 67206-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SQLE antibody (67206-1-Ig, Clone: 1C9A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67206-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50872-2

    Cytometric bead array standard curve of MP50872-2

    Cytometric bead array standard curve of MP50872-2, SQLE Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67206-1-PBS. Detection antibody: 67206-3-PBS. Standard:Ag3266. Range: 0.098-100 ng/mL.

  • Cytometric bead array standard curve of MP50872-3

    Cytometric bead array standard curve of MP50872-3

    Cytometric bead array standard curve of MP50872-3, SQLE Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67206-1-PBS. Detection antibody: 67206-4-PBS. Standard:Ag3266. Range: 0.195-100 ng/mL.

  • Cytometric bead array standard curve of MP50872-4

    Cytometric bead array standard curve of MP50872-4

    Cytometric bead array standard curve of MP50872-4, SQLE Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67206-1-PBS. Detection antibody: 67206-5-PBS. Standard:Ag3266. Range: 0.098-100 ng/mL.

SQLE Monoclonal antibody, PBS Only (Capture)

SQLE Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67206-1-PBS

产品说明书

CloneNo. 1C9A2

宿主/亚型

Mouse / IgG2a

种属反应性

human, rat, pig

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Squalene monooxygenase, squalene epoxidase, ERG1, EC:1.14.14.17, 1C9A2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67206-1-PBS targets SQLE as part of a matched antibody pair:

MP50872-2: 67206-1-PBS capture and 67206-3-PBS detection (validated in Cytometric bead array)

MP50872-3: 67206-1-PBS capture and 67206-4-PBS detection (validated in Cytometric bead array)

MP50872-4: 67206-1-PBS capture and 67206-5-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rat, pig
免疫原 SQLE fusion protein Ag3266 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 squalene epoxidase
别名 Squalene monooxygenase, squalene epoxidase, ERG1, EC:1.14.14.17, 1C9A2
计算分子量 574 aa, 64 kDa
观测分子量 50-64 kDa
GenBank蛋白编号BC017033
基因名称 SQLE
Gene ID (NCBI) 6713
RRIDAB_2882499
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14534
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SQLE, also named as ERG1, SE and SM, belongs to the squalene monooxygenase family. It catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before cyclization into the basic steroid structure. SQLE may serve as a flux-controlling enzyme beyond 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, considered as rate limiting). It is also posttranslationally regulated by cholesterol-dependent proteasomal degradation. SQLE is subject to feedback regulation via cholesterol-induced degradation, which depends on its lipid-sensing N terminal regulatory domain. Truncation of SQLE occurs during its endoplasmic reticulum-associated degradation and requires the proteasome, which partially degrades the SQLE N-terminus and eliminates cholesterol-sensing elements within this region. The MW of SQLE is about 50-64 kDa. (PMID:21356516, PMID: 28972164)