验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse kidney tissue, mouse lung tissue, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue, human lung tissue, human kidney tissue, human pancreas cancer tissue, human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SPTBN1 Ready-To-Use IHC Kit. SPTBN1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19722-1-AP targets SPTBN1-Specific in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | spectrin, beta, non-erythrocytic 1 |
| 别名 | SPTBN1, Beta II spectrin, Beta-II spectrin, Spectrin beta chain, non-erythrocytic 1, Spectrin, non-erythroid beta chain 1 |
| 计算分子量 | 275 kDa |
| 观测分子量 | 275 kDa |
| GenBank蛋白编号 | NM_003128 |
| 基因名称 | SPTBN1 |
| Gene ID (NCBI) | 6711 |
| RRID | AB_10638469 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q01082 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPTBN1 is the non-erythrocytic form of β-spectrin or β-fodrin. Spectrins are members of the superfamily of F-actin cross linking proteins that are important as scaffolding proteins for protein sorting, cell adhesion, and migration. Reduced expression of SPTBN1 has been found to correlate with shorter survival of patients with hepatocellular cancer, pancreatic cancer and other malignancies of the gastrointestinal tract, suggesting tumor suppression ability of this protein.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SPTBN1-Specific antibody 19722-1-AP | Download protocol |
| IP protocol for SPTBN1-Specific antibody 19722-1-AP | Download protocol |
| WB protocol for SPTBN1-Specific antibody 19722-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuron Species-specific FMRP regulation of RACK1 is critical for prenatal cortical development | ||
Theranostics Promotion of Cx26 mutants located in TM4 region for membrane translocation successfully rescued hearing loss | ||
Adv Sci (Weinh) The E3 Ligase RNF115 Aggravates Pathological Cardiac Hypertrophy via Ubiquitin-Mediated Degradation of SPTBN1. | ||
Arterioscler Thromb Vasc Biol Valvular Leaflets Are Not Innocent Bystanders: Divergent Fibrotic Remodeling Accompanies Functional Mitral and Tricuspid Regurgitation | ||
Anal Chem Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes. |
