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验证数据展示

  • WB analysis using 31676-1-AP

    WB analysis using 31676-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31676-1-AP (SPTAN1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 31676-1-AP

    IHC staining of human stomach cancer using 31676-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31676-1-AP (SPTAN1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPTAN1 antibody included
  • WB analysis using 31676-1-AP

    WB analysis using 31676-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31676-1-AP (SPTAN1 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 31676-1-AP

    IHC staining of mouse colon using 31676-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31676-1-AP (SPTAN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPTAN1 antibody included
  • IHC staining of mouse colon using 31676-1-AP

    IHC staining of mouse colon using 31676-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31676-1-AP (SPTAN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPTAN1 antibody included
  • IF Staining of HeLa using 31676-1-AP

    IF Staining of HeLa using 31676-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SPTAN1 antibody (31676-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

SPTAN1 Polyclonal antibody

SPTAN1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31676-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

SPTA2, NEAS, Alpha II spectrin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHT-29 cells, mouse brain tissue, HeLa cells, MDA-MB-231 cells, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SPTAN1 Ready-To-Use IHC Kit. SPTAN1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31676-1-AP targets SPTAN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SPTAN1 fusion protein Ag36472 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)
别名 SPTA2, NEAS, Alpha II spectrin
计算分子量285kDa，2472aa
观测分子量285 kDa,150 kDa
GenBank蛋白编号NM_003127.3
基因名称 SPTAN1
Gene ID (NCBI) 6709
RRIDAB_3670071
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ13813
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPTAN1 (Nonerythroid spectrin αII), also termed α-Fodrin, is a cytoskeletal protein that belongs to the family of spectrins. Spectrins includes several structural proteins (α- and β-spectrin, α-actinin, dystrophin, and utrophin) that build and stabilize the cytoskeleton by forming a hexagonal mesh under the plasma membrane and ensuring stability and organization of organelles in the cell. SPTAN1 encodes for a 2,472 amino acid protein with a predicted molecular weight of 284 kDa and can be cleaved by calpain leading to a 150 kDa fragment (PMID: 31186638).

实验方案

Product Specific Protocols
WB protocol for SPTAN1 antibody 31676-1-APDownload protocol
IHC protocol for SPTAN1 antibody 31676-1-APDownload protocol
IF protocol for SPTAN1 antibody 31676-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols