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验证数据展示

  • WB analysis of Calu-1 using 12194-1-AP

    WB analysis of Calu-1 using 12194-1-AP

    WB result of SPSB1 antibody (12194-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPSB1 transfected Calu-1 cells.

  • WB analysis of K-562 using 12194-1-AP

    WB analysis of K-562 using 12194-1-AP

    WB result of SPSB1 antibody (12194-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPSB1 transfected K-562 cells.

  • WB analysis using 12194-1-AP

    WB analysis using 12194-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12194-1-AP (SPSB1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

SPSB1 Polyclonal antibody

SPSB1 Polyclonal Antibody for WB, ELISA
Cat No. 12194-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

SPRY domain-containing SOCS box protein 1, SSB 1, SSB1, SSB-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, Calu-1 cells, K-562 cells, L02 cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12194-1-AP targets SPSB1 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag2832

Product name: Recombinant human SPSB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-273 aa of BC015711

Sequence: MGQKVTGGIKTVDMRDPTYRPLKQELQGLDYCKPTRLDLLLDMPPVSYDVQLLHSWNNNDRSLNVFVKEDDKLIFHRHPVAQSTDAIRGKVGYTRGLHVWQITWAMRQRGTHAVVGVATADAPLHSVGYTTLVGNNHESWGWDLGRNRLYHDGKNQPSKTYPAFLEPDETFIVPDSFLVALDMDDGTLSFIVDGQYMGVAFRGLKGKKLYPVVSAVWGHCEIRMRYLNGLDPEPLPLMDLCRRSVRLALGRERLGEIHTLPLPASLKAYLLYQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 splA/ryanodine receptor domain and SOCS box containing 1
别名 SPRY domain-containing SOCS box protein 1, SSB 1, SSB1, SSB-1
计算分子量 273 aa, 31 kDa
观测分子量40 kDa
GenBank蛋白编号BC015711
基因名称 SPSB1
Gene ID (NCBI) 80176
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96BD6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPSB1, also known as Single-stranded DNA binding protein 1 (SSB1), is a member of the single-stranded DNA binding protein (SSB) family. It plays a pivotal role in the DNA damage response. As an early responder, SSB1 promptly associates with exposed single-strand DNA to shield it from degradation by various nucleases present in the cellular milieu.

实验方案

Product Specific Protocols
WB protocol for SPSB1 antibody 12194-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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