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SPRYD5 Polyclonal antibody

SPRYD5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20802-1-AP

Print datasheet

Synonyms

SPRY domain containing 5, SPRYD5, TRIM51

验证数据展示

  • WB analysis of Jurkat using 20802-1-AP

    WB analysis of Jurkat using 20802-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 20802-1-AP (SPRYD5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 20802-1-AP

    IHC staining of human placenta using 20802-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20802-1-AP (SPRYD5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 20802-1-AP

    IHC staining of human placenta using 20802-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20802-1-AP (SPRYD5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells, rat brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20802-1-AP targets SPRYD5 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, rat
Immunogen SPRYD5 fusion protein Ag14723 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SPRY domain containing 5
Synonyms SPRY domain containing 5, SPRYD5, TRIM51
Calculated Molecular Weight 293 aa, 34 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC005014
Gene Symbol SPRYD5
Gene ID (NCBI) 84767
RRIDAB_2878743
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BSJ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SPRYD5 antibody 20802-1-APDownload protocol
IHC protocol for SPRYD5 antibody 20802-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols