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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 87666-1-RR (same clone as 87666-1-PBS)

    WB analysis using 87666-1-RR (same clone as 87666-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87666-1-RR (SPRY4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87666-1-PBS in a different storage buffer formulation.

  • IF Staining of A375 using 87666-1-RR (same clone as 87666-1-PBS)

    IF Staining of A375 using 87666-1-RR (same clone as 87666-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A375 cells using SPRY4 antibody (87666-1-RR, Clone: 253045D12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 87666-1-PBS in a different storage buffer formulation.

  • Affinity of 87666-1-RR

    Affinity of 87666-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87666-1-RR against Human SPRY4 were performed. The affinity constant is 1.07 nM.

SPRY4 Recombinant monoclonal antibody, PBS Only

SPRY4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 87666-1-PBS

产品说明书

CloneNo. 253045D12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

HH17, Protein sprouty homolog 4, sprouty homolog 4 (Drosophila), Spry 4, Spry-4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

87666-1-PBS targets SPRY4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18574

Product name: Recombinant human SPRY4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-165 aa of BC125095

Sequence: MEPPIPQSAPLTPNSVMVQPLLDSRMSHSRLQHPLTILPIDQVKTSHVENDYIDNPSLALTTGPKRTRGGAPELAPTPARCDQDVTHHWISFSGRPSSVSSSSSTSSDQRLLDHMAPPPVADQASPRAVRIQPKVVHCQPLDLKGPAVPPELDKHFLLCEACGKC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sprouty homolog 4 (Drosophila)
别名 HH17, Protein sprouty homolog 4, sprouty homolog 4 (Drosophila), Spry 4, Spry-4
计算分子量 299 aa, 33 kDa
观测分子量 35-38 kDa
GenBank蛋白编号BC125095
基因名称 SPRY4
Gene ID (NCBI) 81848
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9C004
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPRY4, also named as Protein sprouty homolog 4, is a 299 amino acid protein, which contains one SPR (sprouty) domain and belongs to the sprouty family. SPRY4 Suppresses the INS receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras. Probably impairs the formation of GTP-Ras. SPRY4 has a cellular function to regulate the actin cytoskeleton, independent of its inhibitory activity on the Ras/MAP kinase signalling. SPRY1, SPRY2, SPRY3, SPRY4, are central and complex regulators of the receptor tyrosine kinase (RTK) signalling pathway.

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