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- KD/KO Validated
SPRY2 Polyclonal antibody
SPRY2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | SW 1990 cells, Jurkat cells, A375 cells, Neuro-2a cells |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A375 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11383-1-AP targets SPRY2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | SPRY2 fusion protein Ag1918 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | sprouty homolog 2 (Drosophila) |
Synonyms | hSPRY2, Protein sprouty homolog 2, sprouty homolog 2 (Drosophila), Spry 2, SPRY2 |
Calculated Molecular Weight | 35 kDa |
Observed Molecular Weight | 35-40 kDa, 60~65 kDa |
GenBank Accession Number | BC015745 |
Gene Symbol | SPRY2 |
Gene ID (NCBI) | 10253 |
RRID | AB_2195287 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O43597 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SPRY2 antibody 11383-1-AP | Download protocol |
IHC protocol for SPRY2 antibody 11383-1-AP | Download protocol |
IF protocol for SPRY2 antibody 11383-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Int J Mol Sci Kub3 Deficiency Causes Aberrant Late Embryonic Lung Development in Mice by the FGF Signaling Pathway. | ||
J Cell Mol Med Exosomal miR-27 negatively regulates ROS production and promotes granulosa cells apoptosis by targeting SPRY2 in OHSS.
| ||
Toxicol Appl Pharmacol Hexachlorophene, a selective SHP2 inhibitor, suppresses proliferation and metastasis of KRAS-mutant NSCLC cells by inhibiting RAS/MEK/ERK and PI3K/AKT signaling pathways. | ||
Cell Biol Int Sprouty2 is involved in the control of osteoblast proliferation and differentiation through the FGF and BMP signaling pathways. | ||
Breast Cancer Res Loss of SPRY2 contributes to cancer-associated fibroblasts activation and promotes breast cancer development
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Cancer Cell Int Therapeutic effect and transcriptome-methylome characteristics of METTL3 inhibition in liver hepatocellular carcinoma |