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验证数据展示

  • WB analysis using 17813-1-AP

    WB analysis using 17813-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17813-1-AP (SPRY1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HT-29 using 17813-1-AP

    IP experiment of HT-29 using 17813-1-AP

    IP result of anti-SPRY1 (IP:17813-1-AP, 4ug; Detection:17813-1-AP 1:3000) with HT-29 cells lysate 1760 ug.

  • IP experiment of A2780 using 17813-1-AP

    IP experiment of A2780 using 17813-1-AP

    IP result of anti-SPRY1 (IP:17813-1-AP, 4ug; Detection:17813-1-AP 1:3000) with A2780 cells lysate 1000 ug.

  • WB analysis using 17813-1-AP

    WB analysis using 17813-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17813-1-AP (SPRY1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

SPRY1 Polyclonal antibody

SPRY1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 17813-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

hSPRY1, Protein sprouty homolog 1, Spry 1, Spry-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA2780 cells, HEK-293 cells, HepG2 cells
Positive IP detected inHT-29 cells, A2780 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17813-1-AP targets SPRY1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12129

Product name: Recombinant human SPRY1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-319 aa of BC063856

Sequence: MDPQNQHGSGSSLVVIQQPSLDSRQRLDYEREIQPTAILSLDQIKAIRGSNEYTEGPSVVKRPAPRTAPRQEKHERTHEIIPINVNNNYEHRHTSHLGHAVLPSNARGPILSRSTSTGSAASSGSNSSASSEQGLLGRSPPTRPVPGHRSERAIRTQPKQLIVDDLKGSLKEDLTQHKFICEQCGKCKCGECTAPRTLPSCLACNRQCLCSAESMVEYGTCMCLVKGIFYHCSNDDEGDSYSDNPCSCSQSHCCSRYLCMGAMSLFLPCLLCYPPAKGCLKLCRRCYDWIHRPGCRCKNSNTVYCKLESCPSRGQGKPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sprouty homolog 1, antagonist of FGF signaling (Drosophila)
别名 hSPRY1, Protein sprouty homolog 1, Spry 1, Spry-1
计算分子量 319 aa, 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC063856
基因名称 SPRY1
Gene ID (NCBI) 10252
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43609
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sprouty homolog 1 (SPRY1) belongs to the sprouty family. Spry-1 is a negative regulator of receptor tyrosine kinase signalling (PMID: 36543535). Spry1 and its related proteins have also been implicated as negative regulators of RhoA and its downstream effector Rho kinase through the non-canonical Wnt signaling (PMID: 19671746, PMID: 15184877). Sprouty-1 and Sprouty-2 proteins are expressed in early embryonic lung both in the epithelial cells of distal tubules and in peripheral mesenchymal cells (PMID: 22236546). Spry1 is a cytoplasmic protein that plays a critical role in kidney development and is primarily known to inhibit the Ras/MEK/ERK pathway (PMID: 21310958).

实验方案

Product Specific Protocols
IP protocol for SPRY1 antibody 17813-1-APDownload protocol
WB protocol for SPRY1 antibody 17813-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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