• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SPOCK1 Polyclonal antibody

SPOCK1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 28203-1-AP

Print datasheet

Synonyms

FLJ37170, Protein SPOCK, SPOCK, SPOCK1, TESTICAN, Testican 1, TIC1, TICN1

验证数据展示

  • WB analysis using 28203-1-AP

    WB analysis using 28203-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28203-1-AP (SPOCK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 28203-1-AP

    IHC staining of human prostate cancer using 28203-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28203-1-AP (SPOCK1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 28203-1-AP

    IHC staining of human prostate cancer using 28203-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 28203-1-AP (SPOCK1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells, DU 145 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28203-1-AP targets SPOCK1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen SPOCK1 fusion protein Ag28213 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sparc/osteonectin, cwcv and kazal-like domains proteoglycan (testican) 1
Synonyms FLJ37170, Protein SPOCK, SPOCK, SPOCK1, TESTICAN, Testican 1, TIC1, TICN1
Calculated Molecular Weight 439 aa, 49 kDa
Observed Molecular Weight49 kDa
GenBank Accession NumberBC030691
Gene Symbol SPOCK1
Gene ID (NCBI) 6695
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08629
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SPOCK1 antibody 28203-1-APDownload protocol
IHC protocol for SPOCK1 antibody 28203-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols