CD43 Polyclonal antibody

CD43 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG







Cat No : 13959-1-AP

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CD43, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, sialophorin, SPN


Positive WB detected inK-562 cells, Jurkat cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD43 Ready-To-Use IHC Kit. CD43 primary antibody included.


Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


13959-1-AP targets CD43 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB,ELISA Application Description
Tested Reactivity human
Immunogen CD43 fusion protein Ag4836 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sialophorin
Synonyms CD43, Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, sialophorin, SPN
Calculated Molecular Weight 400 aa, 43 kDa
Observed Molecular Weight 60-66 kDa
GenBank Accession NumberBC012350
Gene Symbol SPN
Gene ID (NCBI) 6693
ENSEMBL Gene IDENSG00000197471
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.


Product Specific Protocols
WB protocol for CD43 antibody 13959-1-APDownload protocol
IHC protocol for CD43 antibody 13959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols