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验证数据展示

  • WB analysis of K-562 using 13959-1-AP

    WB analysis of K-562 using 13959-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13959-1-AP (CD43 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 13959-1-AP

    IHC staining of human tonsillitis using 13959-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13959-1-AP (CD43 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD43 antibody included
  • IHC staining of human tonsillitis using 13959-1-AP

    IHC staining of human tonsillitis using 13959-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13959-1-AP (CD43 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD43 antibody included

CD43 Polyclonal antibody

CD43 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13959-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Leukosialin, Leukocyte sialoglycoprotein, GPL115, GALGP, Galactoglycoprotein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD43 Ready-To-Use IHC Kit. CD43 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13959-1-AP targets CD43 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4836

Product name: Recombinant human SPN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 101-400 aa of BC012350

Sequence: KMSSVPQETPHATSHPAVPITANSLGSHTVTGGTITTNSPETSSRTSGAPVTTAASSLETSRGTSGPPLTMATVSLETSKGTSGPPVTMATDSLETSTGTTGPPVTMTTGSLEPSSGASGPQVSSVKLSTMMSPTTSTNASTVPFRNPDENSRGMLPVAVLVALLAVIVLVALLLLWRRRQKRRTGALVLSRGGKRNGVVDAWAGPAQVPEEGAVTVTVGGSGGDKGSGFPDGEGSSRRPTLTTFFGRRKSRQGSLAMEELKSGSGPSLKGEEEPLVASEDGAVDAPAPDEPEGGDGAAP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sialophorin
别名 Leukosialin, Leukocyte sialoglycoprotein, GPL115, GALGP, Galactoglycoprotein
计算分子量 400 aa, 43 kDa
观测分子量 60-66 kDa
GenBank蛋白编号BC012350
基因名称 CD43
Gene ID (NCBI) 6693
ENSEMBL Gene IDENSG00000197471
RRIDAB_10694668
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16150
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD43, also named leukosialin, sialophorin, galactoglycoprotein, leukocyte sialoglycoprotein, SPN, and is a mucin-like type I transmembrane protein. In humans, it is expressed in haematopoietic cells, including T lymphocytes, monocytes, granulocytes, natural killer cells, platelets, and haematopoietic stem cells, with the exception of mature erythrocytes and B cell subpopulations. The unglycosylated form of CD43 migrates on SDS-PAGE with a molecular weight of 54 kDa (PMID: 2943740), whereas CD43 from different haematopoietic cell lines displays increased molecular weights since it is glycosylated with O-linked chains that differ in core structure and sialylation.

实验方案

Product Specific Protocols
IHC protocol for CD43 antibody 13959-1-APDownload protocol
WB protocol for CD43 antibody 13959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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