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PBK Polyclonal antibody

PBK Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 16110-1-AP

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Synonyms

Cancer/testis antigen 84, CT84, MAPKK like protein kinase, Nori 3, PBK, PDZ binding kinase, SPK, TOPK



经过测试的应用

Positive WB detected inHeLa cells, mouse ovary tissue, mouse testis tissue, Jurkat cells, NIH/3T3 cells
Positive IHC detected inhuman placenta tissue, human lung cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16110-1-AP targets PBK in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IHC, WB, ELISA
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen PBK fusion protein Ag9033 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PDZ binding kinase
Synonyms Cancer/testis antigen 84, CT84, MAPKK like protein kinase, Nori 3, PBK, PDZ binding kinase, SPK, TOPK
Calculated Molecular Weight 322 aa, 36 kDa
Observed Molecular Weight 36-40 kDa
GenBank Accession NumberBC015191
Gene Symbol PBK
Gene ID (NCBI) 55872
RRIDAB_2283508
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96KB5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PBK(PDZ-binding kinase) is also named as TOPK, CT84, Nori-3, SPK and belongs to the MAP kinase kinase subfamily. PBK may have a role in the regulation of cellular proliferation and progression of the cell cycle. It has a characteristic cdc2ycyclin B phosphorylation site(SyT-P-X-KyR) at its N terminus,which is conserved across species, and it is phosphorylated in a cell cycle-dependent manner at mitosis, and that this phosphorylation is required for its activation(PMID:10779557).

实验方案

Product Specific Protocols
WB protocol for PBK antibody 16110-1-APDownload protocol
IHC protocol for PBK antibody 16110-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Genomics Proteomics Bioinformatics

Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma.

Authors - Yixian Cun
mouseWB,IF

FASEB J

Autophagy plays a positive role in induction of epidermal proliferation.

Authors - Junyi Wang
mouseIF

Invest Ophthalmol Vis Sci

Single-Cell Transcriptomics Reveals Cellular Heterogeneity and Complex Cell-Cell Communication Networks in the Mouse Cornea

Authors - Yueh-Feng Wu
humanIHC

Biomolecules

Identification of a Fibroblast-Related Prognostic Model in Glioma Based on Bioinformatics Methods

Authors - Haofuzi Zhang
mouseWB,IF

Invest Ophthalmol Vis Sci

Single-Cell RNA Transcriptome Helps Define the Limbal/Corneal Epithelial Stem/Early Transit Amplifying Cells and How Autophagy Affects This Population.

Authors - Nihal Kaplan
  • KD Validated
ratWB

Front Endocrinol (Lausanne)

PBK/TOPK Inhibitor Suppresses the Progression of Prolactinomas.

Authors - Kejing Zhu