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验证数据展示

  • WB analysis using 11295-1-AP (same clone as 11295-1-PBS)

    WB analysis using 11295-1-AP (same clone as 11295-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11295-1-AP (SPIRE1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11295-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 11295-1-AP (same clone as 11295-1-PBS)

    IHC staining of human lung cancer using 11295-1-AP (same clone as 11295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11295-1-AP (SPIRE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 11295-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 11295-1-AP (same clone as 11295-1-PBS)

    IHC staining of human lung cancer using 11295-1-AP (same clone as 11295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11295-1-AP (SPIRE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with . This data was developed using the same antibody clone with 11295-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse stomach using 11295-1-AP (same clone as 11295-1-PBS)

    IHC staining of mouse stomach using 11295-1-AP (same clone as 11295-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 11295-1-AP (SPIRE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11295-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 11295-1-AP (same clone as 11295-1-PBS)

    IF Staining of mouse brain using 11295-1-AP (same clone as 11295-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SPIRE1 antibody (11295-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11295-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 11295-1-AP (same clone as 11295-1-PBS)

    IF Staining of mouse brain using 11295-1-AP (same clone as 11295-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using SPIRE1 antibody (11295-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11295-1-PBS in a different storage buffer formulation.

SPIRE1 Polyclonal antibody, PBS Only

SPIRE1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 11295-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

KIAA1135, Protein spire homolog 1, Spir 1, SPIR1, Spir-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11295-1-PBS targets SPIRE1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1798

Product name: Recombinant human SPIRE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 600-756 aa of BC016825

Sequence: GRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESSMRSEKPSTAHHRPLRSIARFSSKSKSMDKSDEELQFPKELMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSPGPSEYCPSERTISEI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 spire homolog 1 (Drosophila)
别名 KIAA1135, Protein spire homolog 1, Spir 1, SPIR1, Spir-1
计算分子量 64 kDa
观测分子量50-60 kDa, 95 kDa
GenBank蛋白编号BC016825
基因名称 SPIRE1
Gene ID (NCBI) 56907
RRIDAB_3085370
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ08AE8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPIRE1, Protein spire homolog 1, belongs to the family of Wiskott-Aldrich homology region-2 (WH2) proteins (PMID: 11747823). SPIRE1 acts as an actin nucleation factor, associated with the slow-growing pointed end of the new filament (PMID: 11747823, PMID: 21620703). SPIRE1 and FMN2 promote assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (PMID: 26287480).

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