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HAI-1 Polyclonal antibody

HAI-1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15036-1-AP

Print datasheet

Synonyms

HAI, HAI 1, HAI1, HAI-1, MANSC2, SPINT1

验证数据展示

  • WB analysis of human placenta using 15036-1-AP

    WB analysis of human placenta using 15036-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 15036-1-AP (HAI-1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 15036-1-AP

    IHC staining of human prostate cancer using 15036-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15036-1-AP (HAI-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 15036-1-AP

    IHC staining of human prostate cancer using 15036-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15036-1-AP (HAI-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NIH/3T3 using 15036-1-AP

    IF Staining of NIH/3T3 using 15036-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 15036-1-AP (HAI-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15036-1-AP targets HAI-1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen HAI-1 fusion protein Ag6665 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serine peptidase inhibitor, Kunitz type 1
Synonyms HAI, HAI 1, HAI1, HAI-1, MANSC2, SPINT1
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC004140
Gene Symbol SPINT1
Gene ID (NCBI) 6692
RRIDAB_2196284
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43278
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HAI-1 antibody 15036-1-APDownload protocol
IHC protocol for HAI-1 antibody 15036-1-APDownload protocol
IF protocol for HAI-1 antibody 15036-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols