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验证数据展示

  • WB analysis of human placenta using 15036-1-AP

    WB analysis of human placenta using 15036-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 15036-1-AP (HAI-1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 15036-1-AP

    IHC staining of human prostate cancer using 15036-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15036-1-AP (HAI-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 15036-1-AP

    IHC staining of human prostate cancer using 15036-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15036-1-AP (HAI-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NIH/3T3 using 15036-1-AP

    IF Staining of NIH/3T3 using 15036-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using HAI-1 antibody (15036-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SPINT1/HAI-1 Polyclonal antibody

SPINT1/HAI-1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15036-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

HAI 1, HAI-1, SPINT1, HAI, HAI1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15036-1-AP targets SPINT1/HAI-1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SPINT1/HAI-1 fusion protein Ag6665 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serine peptidase inhibitor, Kunitz type 1
别名 HAI 1, HAI-1, SPINT1, HAI, HAI1
计算分子量 58 kDa
观测分子量 58-70 kDa
GenBank蛋白编号BC004140
基因名称 HAI-1
Gene ID (NCBI) 6692
RRIDAB_2196284
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43278
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hepatocyte growth factor activator inhibitor type 1 (HAI-1) is also named as SPINT1 and Kunitz-type protease inhibitor 1. HAI-1 is critical to control matriptase proteolytic activity (PMID: 25310042). HAI-1 inhibits matriptase activity through forming complex with activated matriptase (PMID: 30370662). matriptase, HAI‐1 also binds to and inhibits a variety of serine proteases including of hepsin, plasmin, prostasin, TMPRSS13 and HAT. HAI-1 as a cell-autonomous tumor suppressor that negatively regulates the HGF pathway (PMID: 25925948). HAI-1 is inhibitor of HGFAC (PMID: 9045658). HAI-1 inhibits serine protease activity of ST14/matriptase in vitro (PMID: 28710277). HAI-1 inhibits serine protease activity of TMPRSS13, via the BPTI/Kunitz inhibitor 1 domain (PMID: 20977675). HAI-1 expression is reduced in many cancer types, which is implicated in the progression of cancer and is associated with a worse prognosis in cancer patients (PMID: 30370662).

实验方案

Product Specific Protocols
WB protocol for SPINT1/HAI-1 antibody 15036-1-APDownload protocol
IHC protocol for SPINT1/HAI-1 antibody 15036-1-APDownload protocol
IF protocol for SPINT1/HAI-1 antibody 15036-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols