验证数据展示
经过测试的应用
| Positive IHC detected in | rat testis tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24656-1-AP targets SPINK5L3 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag19715 Product name: Recombinant human SPINK5L3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 40-94 aa of BC128164 Sequence: KMYIPLDPDYNADCPNVTAPVCASNGHTFQNECFFCVEQREFHYRIKFEKYGKCD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | serine PI Kazal type 5-like 3 |
| 别名 | HBVDNAPTP1, HESPINTOR, serine PI Kazal type 5 like 3, SPINK13 |
| 计算分子量 | 94 aa, 11 kDa |
| GenBank蛋白编号 | BC128164 |
| 基因名称 | SPINK5L3 |
| Gene ID (NCBI) | 153218 |
| RRID | AB_3742120 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q1W4C9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPINK5L3 (Serine protease inhibitor Kazal-type 13) may be a serine protease inhibitor. It enables serine-type endopeptidase inhibitor activity, which is involved in the negative regulation of the acrosome reaction.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SPINK5L3 antibody 24656-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
