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SPIB Polyclonal antibody

SPIB Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 15768-1-AP

Print datasheet

Synonyms

SPI B, SPIB, Transcription factor Spi B

验证数据展示

  • IHC staining of human liver cancer using 15768-1-AP

    IHC staining of human liver cancer using 15768-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15768-1-AP (SPIB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPIB antibody included
  • IHC staining of human liver cancer using 15768-1-AP

    IHC staining of human liver cancer using 15768-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15768-1-AP (SPIB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SPIB antibody included
  • WB analysis of L02 using 15768-1-AP

    WB analysis of L02 using 15768-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 15768-1-AP (SPIB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of L02 using 15768-1-AP

    IP experiment of L02 using 15768-1-AP

    IP result of anti-SPIB (IP:15768-1-AP, 4ug; Detection:15768-1-AP 1:300) with L02 cells lysate 1800ug.

经过测试的应用

Positive WB detected inL02 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SPIB Ready-To-Use IHC Kit. SPIB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15768-1-AP targets SPIB in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen SPIB fusion protein Ag8404 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Spi-B transcription factor (Spi-1/PU.1 related)
Synonyms SPI B, SPIB, Transcription factor Spi B
Calculated Molecular Weight 262 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC007921
Gene Symbol SPIB
Gene ID (NCBI) 6689
RRIDAB_2878182
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01892
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SPIB antibody 15768-1-APDownload protocol
IHC protocol for SPIB antibody 15768-1-APDownload protocol
IP protocol for SPIB antibody 15768-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Signal

SPIB acts as a tumor suppressor by activating the NFkB and JNK signaling pathways through MAP4K1 in colorectal cancer cells.

Authors - Xunping Zhao
View Article