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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68871-4-Ig (same clone as 68871-4-PBS)

    WB analysis using 68871-4-Ig (same clone as 68871-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68871-4-Ig (SPHK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68871-4-PBS in a different storage buffer formulation.

SPHK2 Monoclonal antibody, PBS Only

SPHK2 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68871-4-PBS

产品说明书

CloneNo. 2B5G5

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

2B5G5, EC:2.7.1.91, SK 2, SK-2, Sphingosine kinase 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SPHK2 Ready-To-Use IHC Kit. SPHK2 primary antibody included.

产品信息

68871-4-PBS targets SPHK2 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10569

Product name: Recombinant human SPHK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 266-618 aa of BC010671

Sequence: LNCSLLLCRGGGHPLDLLSVTLASGSRCFSFLSVAWGFVSDVDIQSERFRALGSARFTLGTVLGLATLHTYRGRLSYLPATVEPASPTPAHSLPRAKSELTLTPDPAPPMAHSPLHRSVSDLPLPLPQPALASPGSPEPLPILSLNGGGPELAGDWGGAGDAPLSPDPLLSSPPGSPKAALHSPVSEGAPVIPPSSGLPLPTPDARVGASTCGPPDHLLPPLGTPLPPDWVTLEGDFVLMLAISPSHLGADLVAAPHARFDDGLVHLCWVRSGISRAALLRLFLAMERGSHFSLGCPQLGYAAARAFRLEPLTPRGVLTVDGEQVEYGPLQAQMHPGIGTLLTGPPGCPGREP

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 sphingosine kinase 2
别名 2B5G5, EC:2.7.1.91, SK 2, SK-2, Sphingosine kinase 2
计算分子量 618 aa, 65 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC010671
基因名称 SPHK2
Gene ID (NCBI) 56848
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NRA0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPHK2(Sphingosine kinase 2) is also named as SK2, SPK2 and catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. SPHK2 associates with HDAC1 and HDAC2 in repressor complexes and is selectively enriched at the promoters of the genes encoding the cyclin-dependent kinase inhibitor p21 or the transcriptional regulator c-fos, where it enhanced local histone H3 acetylation and transcription.SphK2 is a ~66kDa protein that is most abundantly expressed in the liver and heart(PMID:19168577). SphK2 has two splice variants, which are the 68-kDa SphK2-S and the NH2-terminal extended 72-kDa SphK2-L (PMID:17974990).